Radiation-induced changes in the cytoplasmic acridine orange staining of exfoliated cells
✍ Scribed by H. Gunter Seydel
- Publisher
- John Wiley and Sons
- Year
- 1965
- Tongue
- English
- Weight
- 361 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
T (a.0.) stained smears of exfoliated cells from human cancers has been evaluated critically by Berta1anffy.l He presented only limited information regarding the use of the stain in patients receiving radiotherapy for accessible malignancies. I feel that the a.0. technique, which is based on the specific binding of the fluorochrome to nucleic acids, offers not only a valuable method in the cytodiagnosis of cancer but also a means of following the progressive radiation-induced changes in these acids in desquamated cells. A description of the alterations in the a. 0. staining of the cytoplasm of irradiated Ehrlich ascites tumor cells and a discussion of the mechanism of the binding of a. 0. in the cytoplasm were reported recently.8 The present paper deals with the effect of irradiation upon the staining properties of the cytoplasmic RNA of malignant cells seen in smears from cancers of the human uterus and oral cavity. The effect of irradiation on the cytoplasmic stain of normal epithelial cells of the vaginal and oral cavity also will be considered.
METHODS AND MATERIALS
Weekly smears of exfoliated cells were obtained from patients undergoing radiation therapy for cancer of the uterus and oral cavity at Detroit Memorial Hospital. The first smear was obtained before the therapy began and the last one was taken 2 weeks after the end of treatment. In some cases the smears ~