Acid phosphatase from psoriatic scales
✍ Scribed by F. J. Förster
- Book ID
- 104810589
- Publisher
- Springer-Verlag
- Year
- 1974
- Tongue
- English
- Weight
- 338 KB
- Volume
- 250
- Category
- Article
- ISSN
- 0340-3696
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📜 SIMILAR VOLUMES
A laboratory process for the isolation of type B acid phosphatase from human erythrocytes has been scaled up 80 times. The enzyme was purified 1300-fold by adsorption on calcium phosphate gel, ammonium sulfate precipitation, and gel filtration. The yield was 277,. Results for the type A enzyme are a
Tris-HC1 buffer containing ethylenediaminetetraacetatic acid (EDTA) and 2-mercaptoethanol (ME), agglutinated erythrocytes (E) sensitized with IgG antibodies (A) (EA), but not E or E sensitized with F(ab')2-fragments of IgG. The agglutination was inhibited by IgG and Fc fragments of IgG, but not by I