We have examined the sequence dependence of aspartimide formation during Fmoc-based solid-phase synthesis of the peptide Val-Lys-Asp-X-Tyr-Ile. The extent of aspartimide formation and subsequent conversion to the c~or [3-piperidide was characterized and quantitated by analytical reversed-phase high-
Acid-Mediated Prevention of Aspartimide Formation in Solid Phase Peptide Synthesis
✍ Scribed by Michels, Tillmann; Dölling, Rudolf; Haberkorn, Uwe; Mier, Walter
- Book ID
- 117994894
- Publisher
- American Chemical Society
- Year
- 2012
- Tongue
- English
- Weight
- 252 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1523-7060
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## Abstract The sequence‐dependent, acid‐ or base‐catalysed aspartimide formation is one of the most serious side reactions in solid‐phase synthesis of peptides containing aspartic acid. In the present work, we investigated the susceptibility of 4‐{__N__‐[1‐(4,4‐dimethyl‐2,6‐dioxocyclohexylidene)‐3
This paper discusses the application of a method developed for cyclic peptide synthesis using allyl-based sidechain-protecting groups to obtain a so-called tailed cyclic peptide, a cyclic peptide bearing a side-chain anchoring tail. The method used for the synthesis ofcyclo[-D-Val-Arg-Gly-Asp-Asp(-e