Accuracy of Cerenkov measurements using a liquid scintillation spectrometer
โ Scribed by Makoto Takiue; Takashi Natake; Haruo Fujii; Tamaru Aburai
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 275 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0969-8043
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โฆ Synopsis
Cerenkov counting efficiency varies with colour quenching and sample turbidity. The activity in a plastic vial can be determined accurately with a colour quenching correction technique, regardless of the presence or absence of turbidity in a sample. On the other hand, the error of the measured activity in a glass vial becomes large with increasing sample turbidity due to the dissimilarity of the quench correction curves for non-turbid and turbid samples.
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A single-step assay for serum glucose measurements is described. The assay is based on the phosphorylation of D-glucose by glucokinase and the measurement of ATP consumption by firefly luciferase. The luminescence is recorded in an ordinary liquid scintillation spectrometer. The use of stable reagen