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Measurements of serum glucose using the luciferin/luciferase system and a liquid scintillation spectrometer

✍ Scribed by Lars-Åke Idahl; Per-Erik Sandström; Janove Sehlin

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 394 KB
Volume: 155
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(86)90243-5

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✦ Synopsis

A single-step assay for serum glucose measurements is described. The assay is based on the phosphorylation of D-glucose by glucokinase and the measurement of ATP consumption by firefly luciferase. The luminescence is recorded in an ordinary liquid scintillation spectrometer. The use of stable reagents and a stable final signal (light emission) makes it possible to analyze a large number of samples in each assay run. The assay is of particular value when repeated serum glucose determinations are performed on samples from small laboratory animals.

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