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Accumulation of arsenic(III) by chlorella vulgaris

✍ Scribed by A. Taboada-de la Calzada; M. C. Villa-Lojo; E. Beceiro-González; E. Alonso-Rodríguez; D. Prada-Rodríguez


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
108 KB
Volume
13
Category
Article
ISSN
0268-2605

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✦ Synopsis


Arsenic occurs naturally in the environment and also through agricultural and industrial pollution. Since arsenic species show different toxicities, it is important to be able to separate them. Methods using microorganisms are being applied increasingly to remove metal ions and different metal species from aqueous solutions. Accumulation of As(III) by Chlorella vulgaris algae was studied, including various factors that influence on accumulation capacity, e.g. pretreatment of the algae (live, dry and lyophilized algae), temperature (4, 22, 37 and 100 °C), pH and exposure time of the algae to arsenic solutions. The pH appears to be the most critical factor, probably due to the species presenting different charges with pH variation. For arsenic species determination, hydride generation atomic absorption spectrometry (HG-AAS) was employed.


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Arsenic accumulated in living Chlorella vulgaris cells was solvent-fractionated with chloroform/ methanol (2: l), and the fractions were analyzed for arsenic. A large part of the accumulated arsenic was localized in the extract residues. The extract residue from the same extraction of C. vulgaris,