The association mode of arsenic accumulated in the freshwater alga Chlorella vulgaris

✍ Scribed by Shigeru Maeda; Hiroshi Arima; Akira Ohki; Kensuke Naka

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 393 KB
• Volume: 6
• Category: Article
• ISSN: 0268-2605
• DOI: 10.1002/aoc.590060414

✦ Synopsis

Arsenic accumulated in living Chlorella vulgaris cells was solvent-fractionated with chloroform/ methanol (2: l), and the fractions were analyzed for arsenic. A large part of the accumulated arsenic was localized in the extract residues.

The extract residue from the same extraction of C. vulgaris, which had been, however, cultured in any arsenic-free Detmer medium (MD), adsorbed arsenic physico-chemically at a concentration of 1.1 mg As g-' dry weight.

Arsenic was found to be combined with protein with molecular weight around 3000 in the arsenicaccumulated living cells. The arsenic-bound protein was analyzed for amino acids. The experimental results showed that no metallothionein-like protein was inductively biosynthesized in C. vulgaris on the exposure to arsenic.