It is well established that the zonae pellucidae of mature unfertilized eggs have the ability to induce the acrosome reaction of capacitated spermatozoa. To determine if this capacity of the zona is species-specific, hamster spermatozoa were allowed to attach to the zonae of homologous and heterolog
Acceleration of acrosome reaction in hamster spermatozoa by lysolecithin
β Scribed by Ohzu, Eiji ;Yanagimachi, Ryuzo
- Book ID
- 102337289
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 392 KB
- Volume
- 224
- Category
- Article
- ISSN
- 0022-104X
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β¦ Synopsis
Abstract
When hamster spermatozoa were preincubated for 1β2 hr in Ca^2+^βcontaining medium and then exposed to lysolecithin (LC), large proportions of the spermatozoa underwent acrosome reactions within 15 min. These acrosomeβreacted spermatozoa were capable of fertilizing eggs. The earliest visible sign of the reaction, wrinkling of the acrosomal cap, seen by 30 sec after exposure to LC. The reaction in many spermatozoa was completed within the next 30 sec.
π SIMILAR VOLUMES
## Abstract Two inhibitors of somatic cell transmethylation, homocysteine thiolactone (Hcy) and 3βdeazadenosine (3βDZA), inhibited the acrosome reactions of hamster sperm in vitro. Hcy (250 ΞΌM) or 3βDZA (200 ΞΌM) inhibited acrosome reactions by approximately 30% after 4 hours of incubation with wash