𝔖 Bobbio Scriptorium
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Abstracts of the Immunocytochemistry Club Meeting on Quantitation in Immunocytochemistry

Publisher
Springer
Year
1985
Tongue
English
Weight
406 KB
Volume
17
Category
Article
ISSN
0018-2214

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Removal of background label in immunocyt
Removal of background label in immunocytochemistry with the apolar lowicryls by using washed protein A-gold-precoupled antibodies in a one-step procedure
✍ DΓΌrrenberger, Markus B. πŸ“‚ Article πŸ“… 1989 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 946 KB

Antiserum mixed with protein A-gold produces aggregates that can be washed by centrifugation and redissociated by ultrasonication. These precoupled antibodies are used for immunocytochemical labeling in a one-step procedure and reveal a very low background on sections of material embedded in polar a