✦ LIBER ✦
Removal of background label in immunocytochemistry with the apolar lowicryls by using washed protein A-gold-precoupled antibodies in a one-step procedure
✍ Scribed by Dürrenberger, Markus B.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1989
- Tongue
- English
- Weight
- 946 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0741-0581
No coin nor oath required. For personal study only.
✦ Synopsis
Antiserum mixed with protein A-gold produces aggregates that can be washed by centrifugation and redissociated by ultrasonication. These precoupled antibodies are used for immunocytochemical labeling in a one-step procedure and reveal a very low background on sections of material embedded in polar and apolar Lowicryls. Controls have shown that the background label stems from serum components with only one protein A binding site and is discarded during the wash steps of the precoupling procedure.