Abstracts of presentations at the Bert Schram Young Investigators' Mini-Symposium

• Publisher: Wiley (John Wiley & Sons)
• Year: 2005
• Tongue: English
• Weight: 100 KB
• Volume: 80
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.20319

✦ Synopsis

Phosphorylation is a central mechanism of cell regulation, and the dynamic interplay between kinases and phosphatases determines the phosphorylation state of a protein (2 or 3) within living systems. The caged phosphoprotein or peptide 1 gives researchers spatial and temporal control over the release of the phosphorylated species 2, yielding a new tool to elucidate its real time functional role. Two synthetic methods for caged phosphopeptides have been developed [1], and nonsense codon suppression has been adapted to incorporate caged phospho-amino acids into full length protein [2]. A validation study demonstrates the utility of these tools where classic genetic approaches cannot provide information [3].