Abstracts of Presentations at the Bert Schram Young Investigators' Mini-Symposium

• Publisher: Wiley (John Wiley & Sons)
• Year: 2003
• Tongue: English
• Weight: 73 KB
• Volume: 71
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.10452

✦ Synopsis

The causes of Multiple Sclerosis (MS) remain obscure. Immunomudulating therapies seem to be the goal of the New Millennium. In particular, immuno-affinity separation is one of the most promising applications. It is based on the removal of Abs or Ags by immobilization of the corresponding Ag or Ab to various matrices. Synthetic peptides to specifically remove Abs, have not been yet so much used as affinity ligands. Since CSF114(Glc) is the first synthetic antigen able to detect high Ab titre in MS patients sera, we developed an immuno-affinity chromatography based on the use of this glycopeptide. We demonstrated that our glycopeptide affinity column is an useful tool for a very convenient one-step purification of specific Abs directly from MS patient sera. By this technique, we characterized the isolated Abs. In particular, we were able to separate IgM from IgG Abs. In order to develop a selective technology for an innovative therapeutic apheresis of MS, different biocompatible solid supports were used in this immuno-affinity chromatography technique.

LY 2 DESIGN, SYNTHESIS AND ANALYSIS OF SINGLE CHAIN RELAXIN MIMETICS