Abstract of nucleic acid and the feulgen reaction : J. O. Ely and M. H. Ross (Anat. Record, 104: 103, 1949)

• Book ID: 103075472
• Publisher: Elsevier Science
• Year: 1950
• Tongue: English
• Weight: 89 KB
• Volume: 249
• Category: Article
• ISSN: 0016-0032
• DOI: 10.1016/0016-0032(50)90023-8

✦ Synopsis

Lack of Depolymerase Effect on Desoxyribonucleic Acid in Living Cells.-J. 0. ELY AND M. H. Ross (Science, 109: 367, April 8, 1949). Desoxyribonucleic acid depolymerase had been reported bq several investigators to remove the ultraviolet light absorbing constituents and the Feulgen stainable material from nuclei in sections of tissue and from the nuclei of dead cells. There had been no reference, so far as these authors are aware, of the use of this enzyme on living cells.

Desoxyribonucleic acid depolymerase did not act on the nuclei of living chicken erythrocytes or of living cells of the Walker Carcinoma No. 256 of the rat; the enzyme acted on these cells after they were killed. Lack of effect of the enzyme on living cells apparently was not because of adsorption of the enzyme or antienzyme activity, but may have been because of cell membrane impermeability or inability of the enzymch to attack nucleic acid in the state that it exists in the living cell. Abstract of Effects of Repeated Low Doses of Neutrons on the Estrous Cycle of the White Rat.-JAMES L. LEITCH, DOUGI,AS Lf. GXY.