Abstract of effects of a protein-free diet on the alkaline and acid phosphatase activity of the liver of the rat : J. O. Ely and M. H. Ross (Nature, 168, 323 (1951))
- Publisher
- Elsevier Science
- Year
- 1951
- Tongue
- English
- Weight
- 46 KB
- Volume
- 252
- Category
- Article
- ISSN
- 0016-0032
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✦ Synopsis
Highly polymerized ribonucleic acid has been prepared from calf and rat liver. The procedure involves the maintenance of the pH between 6 and 7, the precipitation of a nucleic acid-protein complex from the cytoplasmic extract according to the method of Volkin and Carter, heat denaturation of the protein for 2 minutes at 100 °, and removal of the protein by shaking with chloroform.
The average apparent molecular weight of the RNA of a typical preparation, calculated from the sedimentation-velocity constant, was 264,000, corrected for viscosity of the solvent, and 580,000, corrected for viscosity of the solution. The axial ratio was 70:1, and the intrinsic viscosity was 319. Anomalous viscosity and moderate streaming birefringence were exhibited by the RNA solutions.
The RNA was depolymerized by x-radiation, chemically generated free radicals, and sonic vibration.