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Absence of RSV production in enucleated RSV-transformed virogenic mammalian cells fused with chick embryo fibroblasts

✍ Scribed by L. Donner; L. Turek; J. Svoboda

Publisher: John Wiley and Sons
Year: 1974
Tongue: French
Weight: 349 KB
Volume: 14
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910140513

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✦ Synopsis

Abstract

Cells of an RSV‐transformed virogenic Chinese hamster line, RSCH, were enucleated by combining the actions of high g forces and cytochalasin B, and fused with RIF‐free chick embryo fibroblasts using beta‐propiolactone‐inactivated Sendai virus. Contrary to nucleate RSCH cells, enucleated RSCH cells did not produce RSV after fusion. This finding supports the view that proviral RSV DNA is not fully transcribed in a virogenic cell.

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## Abstract The rescue of RSV from the “poorly” virogenic RSV‐transformed rat lines LW13‐RsK1 and LW13‐RsK4 was achieved only after treatment with 5‐bromodeoxyuridine (BUdR) before fusion with chicken embryo fibroblasts. Treatment of these lines with mitomycin C (MC) before fusion did not result in