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Absence of high levels of DNA polymerase α activity in the nuclear matrix prepared from mouse erythroleukemia cells

✍ Scribed by Alberto M. Martelli; Luca M. Neri; Anna Maria Billi; Renato Bareggi; Lucio Cocco

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
453 KB
Volume
17
Category
Article
ISSN
1065-6995

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✦ Synopsis

Abstract

We have examined the association of DNA polymerase α activity with the nuclear matrix prepared by different techniques from mouse erythroleukemia cells. At variance with the data obtained using other cell types we have found that only a small amount (less than 2%) of nuclear DNA polymerase α activity resisted extraction with high‐ionic strength buffers, even if nuclei were heat‐stabilized by incubation at 37°C for 45 min prior to subfractionation. The recovery of DNA polymerase α activity bound to the matrix was unaffected by the type of extracting agent used (NaCl or (NH~4~)~2~ SO~4~), by the extraction sequence or by the method employed for obtaining nuclei. These results could indicate that in some types of cells the nuclear matrix is not involved in DNA replication.

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