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Aberrations of the p53 and RB tumor suppressor genes in squamous cell carcinomas of the lung

Book ID
122363852
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
284 KB
Volume
10
Category
Article
ISSN
0169-5002

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Aberrations of tumor-suppressor genes (p
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Loss of heterozygosity at 4 tumor-suppressor gene loci (pS3, apc, mcc and Rb) was investigated using polymerase chain reactions, in 49 esophageal squamous-cell carcinoma specimens from patients who had undergone curative resection. Mutations in the p53 gene within exons 5 to 8 were also examined. LO

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In this study, we analyzed 10 human squamous cell carcinomas (SCCs) for alterations in the p53 tumor suppressor gene in exons 4 through 9 by single-strand conformation polymorphism (SSCP) analysis. We found that 2 of 10 SCCs displayed unusual SSCP alleles at exon 7 of the p53 gene. Subsequent clonin