Aberrant Expression of DNA Methyltransferases DNMT1 and DNMT3A in Endometriosis
โ Scribed by S. Guo; Y. Wu; E. Strawn; Z. Basir; G. Halverson
- Book ID
- 116467775
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 47 KB
- Volume
- 84
- Category
- Article
- ISSN
- 1556-5653
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## Abstract To explore the role of DNA methylation in the brain, we examined the expression pattern of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the mouse central nervous system (CNS). By comparing the levels of Dnmt3a and Dnmt3b mRNAs and proteins in the CNS, we showed that Dnmt3b is det
## Abstract De novo DNA methyltransferases, Dnmt3a and 3b, were purified by fractionation of Sโ100 extract from mouse lymphosarcoma cells through several chromatographic matrices followed by glycerol density gradient centrifugation. Dnmt3a was separated from Dnmt3b and Dnmt1 in the first column, Qโ