A rapid and simple gas-liquid chromatographic method for the determination of subnanomolar amounts of carbohydrates derived from glycoproteins is described. The procedure involves methanolysis in the presence of methyl acetate followed by removal of hydrogen chloride by c~va~ration with t-butyl alco
A window diagram for key component analysis in on-line gas chromatography
โ Scribed by Villalobos, Richard
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 407 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0935-6304
No coin nor oath required. For personal study only.
โฆ Synopsis
Abstract
Window diagrams that optimize for the separation of only one or a few components in a complex mixture are applied to onโline process analysis when speed of analysis is more important than a complete separation of all components in the sample. A window diagram based on retention indexes is the most useful for quickly evaluating the feasibility of a given pair of phases. The one with the most useful output is one based on partition ratios, as this can be used directly with the columns in hand. A PCโbased spreadsheet program with integral specific retention volume data for the common liquid phases is described as a tool for selecting the optimum ratio of lengths for the columns in hand.
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