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A rapid and sensitive method for the analysis of carbohydrate components in glycoproteins using gas-liquid chromatography

✍ Scribed by Martin F. Chaplin

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
490 KB
Volume
123
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid and simple gas-liquid chromatographic method for the determination of subnanomolar amounts of carbohydrates derived from glycoproteins is described. The procedure involves methanolysis in the presence of methyl acetate followed by removal of hydrogen chloride by c~va~ration with t-butyl alcohol and trimethylsilylation. The method is also apphcable to samples containing uranic acids and lipids.

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