A Visual High-Sensitivity Microarray for Detection of Hepatitis B Virus DNA and Its Single Nucleotide Polymorphisms
✍ Scribed by Neng-Li ZOU; Yu-Cui ZANG; Hong-Ju MAO; Guo-Qiang ZHAO; Qing-Hui JIN; Jian-Long ZHAO; Yuan-Sen XU
- Book ID
- 119635604
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- Chinese
- Weight
- 329 KB
- Volume
- 40
- Category
- Article
- ISSN
- 1872-2040
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Factors determining the sensitivity and specificity of the spot-blot hybridization technique for the detection of hepatitis B virus DNA in serum were systematically investigated. Methods for pretreatment of serum samples, mode of application of the samples to the transfer membranes, blot treatment a
## Abstract Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) serves as a template for viral replication and plays a role in persistence of HBV infection. The origin and significance of cccDNA in plasma however, is not well understood. A sensitive, specific, and reproducible real‐time