Improved methods for the activation and assay of D-ribulose-1,5-bisphosphate carboxylase and oxygenase are described. The importance of fully activating the enzyme before starting either reaction is emphasized. The enzyme is activated by preincubation with 20 mM M&l, and 10 mM NaHCO,, pH 8.6. To avo
A visual-electrophoretic method for following the purification of ribulose-1,5-bisphosphate carboxylase oxygenase
✍ Scribed by L. Peñarrubia; J. Moreno; P. Carrasco
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 386 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0307-4412
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A mutant of the small subunit of ribulose-l,5-bisphosphate carboxylase/oxygenase (Rubisco), in which Arg53 is replaced by Glu, was synthesized and imported into isolated chloroplasts. The mutant protein was efficiently imported into the chloroplast and correctly processed to the mature size. Like th
The structure and transcription of the genes for the large (LS) and small subunits (SS) of ribulose-l,5-bisphosphate carboxylase/oxygenase of Anacystis nidulans 6301 were studied. The SS gene is located 93 base pairs (bp) downstream from the LS gene on the same DNA strand. Northern hybridization ana