A Versatile Microassay for Elastase Using Succinylated Elastin
โ Scribed by Srinivasa K. Rao; Mahesh Mathrubutham; Alexis Karteron; Keld Sorensen; Jon R. Cohen
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 112 KB
- Volume
- 250
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A method for the determination of elastolytic activity is described. The method employs soluble elastin radiolabeled with either lz51 or 3H coupled to Sepharose beads. The assay can detect as little as 1 rig/ml of elastase. Several different techniques have been employed for the quantitation of ela
A procedure to quantitate trace amounts of elastase in tissue or cell homogenate preparations is described. The procedure is a modification of a method employing NaB3H,-reduced elastin and it does not restrict assay volume. The assay is specific and can distinguish between pancreatic elastase and tr
Quantitation of proteolytic enzyme concentration can be accomplished by measuring the release, due to primary enzyme catalysis, of a second enzyme bound to a particulate substrate. As the primary enzyme acts on the substrate, release of the indicator enzyme into the surrounding medium occurs, which