𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A useful thin-layer chromatography method for the determination of cytidine aminohydrolase activity in serum

✍ Scribed by L.T. Mulligan Jr.; L.B. Mellett

Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
483 KB
Volume
43
Category
Article
ISSN
1873-3778

No coin nor oath required. For personal study only.

✦ Synopsis

A thin-layer chromatography method for the determination of serum cytidine aminohydrolase activity is presented. The method utilizes a tungstic acid deproteinization reagent and the use of new precoated thin-layer sheets to facilitate separation of substrates from their metabolite products. The method has been found to be useful in the separation of numerous pyrimidines and related analogs from both serum and aqueous solutions.

πŸ“œ SIMILAR VOLUMES

A radiometric method for the determinati
A radiometric method for the determination of choline acetylase activity based on thin-layer chromatography
✍ Zoran GrubiΔ‡; TomazΛ‡ Kiauta; Miro Brzin πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 285 KB

A rapid thin-layer chromatographic method on Gelman I.T.L.C. type SA plates for the determination of choline acetylase activity has been developed. The mobile phase consists of sodium tetraphenylboron dissolved in acetonitrile. The Rt values for acetyl coenzyme A and acetate are about 0.1, while tha

A new method for the determination of sa
A new method for the determination of saccharin based on thin-layer chromatography and spectrophotometry
✍ Vidaud, Z. E. ;GarcΓ­a RochΓ©, M. O. ;GonzΓ‘les, Esther πŸ“‚ Article πŸ“… 1987 πŸ› John Wiley and Sons 🌐 English βš– 201 KB πŸ‘ 1 views

Saccharin is extracted from the acidified liquid food with chloroform. This solvent is evaporated ; the residue is dissolved in ethyl acetate and applied on a silica gel GF,,, plate. After chromatographic separation, the saccharin band is scraped off and extracted with 1 % sodium carbonate solution.