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A unique activity assay for carboxypeptidase A in human serum

✍ Scribed by Lynn M. Peterson; Barton Holmquist; J.L. Bethune

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
624 KB
Volume
125
Category
Article
ISSN
0003-2697

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✦ Synopsis

Measurement of carboxypeptidase A, one of the pancreatic proteolytic enzymes, in human serum is made possible by a combination of affinity chromatography to isolate and concentrate the enzyme followed by monitoring activity spectrophotometrically with a high-turnover pep tide substrate. Concentrations of enzyme in the nanogram-per-milliliter range can be determined with high precision and reliability. Initial clinical application of this method demonstrates no detectable activity in serum from normal individuals, but the enzyme is present in the sera of individuals with pancreatitis.

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