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A type II restriction endonuclease of Streptococcus thermophilus ST117

✍ Scribed by Daniel K.Y. Solaiman; George A. Somkuti


Book ID
109317085
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
380 KB
Volume
80
Category
Article
ISSN
0378-1097

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Restriction of bacteriophage T3 and T7 o
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When E. coli cells carrying the plasmid pLG13 (coding for the newly discovered type II restriction endonuclease EcoRV) are infected with phage T3 or T7, only T7 is able to replicate normally. T3 wild-type as well as its ocr- mutants are subject to DNA restriction in vivo and in vitro. The EcoRV enzy