A two-step enzymatic synthesis of dipeptides
β Scribed by A. Schwarz; C. Wandrey; D. Steinke; M. R. Kula
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 618 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0006-3592
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## Abstract A cascade of two enzymatic transformations is employed in a oneβpot synthesis of cephalexin. The nitrile hydratase (from __R. rhodochrous__ MAWE)βcatalyzed hydration of Dβphenylglycine nitrile to the corresponding amide was combined with the penicillin G acylase (penicillin amidohydrola
V = 1078.3(5) x lo6 pm3, psalsd = 1.089 g ~m -~, Z = 2, crystal size 0.3 x 0.3 x 0.9 mm3, 173 K, Cu,,, radiation, Enraf-Nonius CAD4, scan width 2.25 <8 < 76.5",4202 symmetry-independent and significant reflections [IF1 >3u(F)] structure solution by direct methods (SHELXS-86 [6]) supplemented by diff