A two-stage enzymatic method for determination of uric acid and hypoxanthine/xanthine
β Scribed by S.K. Cunningham; T.V. Keaveny
- Book ID
- 115822097
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 316 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0009-8981
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We describe a sensitive, reproducible method for the simultaneous determination of the ATP catabolites inosine, hypoxanthine, xanthine, and uric acid in biological samples and organ perfusate using reverse-phase chromatography and multiwavelength detection at 254, 270, and 292 nm. Sample preparation
The construction and response of an immobilized enzyme modified electrode as an amperometric sensor is described. Xanthine oxidase was adsorbed on a carbon paste electrode and physically entrapped with a semipermeable membrane. Uric acid, the product of the enzymatic reaction, was oxidized electroc