A twin titration microcalorimeter for the study of biochemical reactions

✍ Scribed by I.R. McKinnon; L. Fall; A. Parody-Morreale; S.J. Gill

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 946 KB
• Volume: 139
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90398-1

✦ Synopsis

A small-volume (200 ~1) titration calorimeter of high sensitivity (1 peal) has been developed for the purpose of studying biochemical reactions where the amounts of material are limited to a few nanomoles. High sensitivity is achieved by calorimetric twining, use of glass cells, elimination of vapor space, effective low-energy stirring, and reduction of measurement time. The calorimeter operates using the heat conduction principal with computer-controlled electrical compensation, which reduces the measurement time of each point from 10 to 3 min. This reduction in time is accompanied by a corresponding increase in the precision of measurement. The use of the calorimeter is demonstrated by a measurement of the heat of oxygenation of hemocyanin.