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A Tryptophanyl-tRNA Synthetase/tRNA Pair for Unnatural Amino Acid Mutagenesis in E. coli

✍ Scribed by Chatterjee, Abhishek; Xiao, Han; Yang, Peng-Yu; Soundararajan, Gautam; Schultz, Peter G.

Book ID
120317968
Publisher
John Wiley and Sons
Year
2013
Tongue
English
Weight
654 KB
Volume
52
Category
Article
ISSN
0044-8249

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Site-specific mutagenesis of DNA, developed some thirty years ago, has proven to be one of the most important advances in biology. By allowing the site-specific replacement of any amino acid in a protein with one of the other nineteen amino acids, it ushered in the new era of "Protein Engineering".