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A time-resolved immunofluorometric assay for quantification of the bovine collectin conglutinin

✍ Scribed by T Krogh-Meibom; U Holmskov; P Løvendahl; N.I Nielsen; K.L Ingvartsen

Book ID
113716736
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
338 KB
Volume
286
Category
Article
ISSN
0022-1759

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We have developed a competitive assay to measure the estrogen-regulated protein pS2. A monoclonal pS2 antibody (mAb) and a biotinylated pS2 peptide are used, with time-resolved fluorometry as a detection technique. The assay has a detection limit of 16 ng/mL and is precise (within-run and day-to-day