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Development of a time-resolved immunofluorometric assay for quantitation of mucosal and systemic antibody responses

✍ Scribed by Martha L Hale; Thomas A Campbell; Yvette G Campbell; Steven E Fong; Bradley G Stiles

Book ID
114050839
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
152 KB
Volume
257
Category
Article
ISSN
0022-1759

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We have developed a competitive assay to measure the estrogen-regulated protein pS2. A monoclonal pS2 antibody (mAb) and a biotinylated pS2 peptide are used, with time-resolved fluorometry as a detection technique. The assay has a detection limit of 16 ng/mL and is precise (within-run and day-to-day