𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A thin layer chromatography laboratory experiment of medical importance

✍ Scribed by Loretta Sharma; Ankur Desai; Ajit Sharma

Publisher
The American Society for Biochemistry and Molecular Biology
Year
2006
Tongue
English
Weight
126 KB
Volume
34
Category
Article
ISSN
1470-8175

No coin nor oath required. For personal study only.

✦ Synopsis

A thin layer chromatography experiment of medical importance is described. The experiment involves extraction of lipids from simulated amniotic fluid samples followed by separation, detection, and scanning of the lecithin and sphingomyelin bands on TLC plates. The lecithin-to-sphingomyelin ratio is calculated. The clinical significance of this number is discussed. Since this is a procedure that is often performed in medical laboratories, most of the supplies and materials for the experiment are commercially available in kit form.

πŸ“œ SIMILAR VOLUMES

A study of the cyclodextrin complexes of
A study of the cyclodextrin complexes of flavonoids by thin layer chromatography
✍ K. Hostettmann; M. Lederer; A. Marston; E. Leipzig-Pagani πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 89 KB πŸ‘ 1 views

The complexation of various flavonoids with cyclodextrins and cyclodextrin derivatives was studied using thin layer chromatography on cellulose. The results indicate that the flavonoids examined do not complex readily with ␣-cyclodextrin or with succinylated ␣-cyclodextrin. However, most do complex

Determination of poly(oxypropylene diol)
Determination of poly(oxypropylene diol) present in poly(oxypropylene triol) by thin-layer chromatography
✍ Walter A. Mameniskis πŸ“‚ Article πŸ“… 1970 πŸ› John Wiley and Sons 🌐 English βš– 325 KB

## Abstract A thin‐layer chromatographic method has been developed for the quantitative determination of poly(oxypropylene diols) present in poly(oxypropylene triols). The polyols are separated on silica gel plates in a modified developing chamber using ethyl acetate as the developer. Quantitative