## Abstract Ruthenium red (RR) is known to be an inhibitor that binds to Ca^2+^ sites. It releases Ca^2+^ and Cl^β^ together with the extrinsic polypeptide of 17 kDa associated with the oxygen evolving complex of photosystem II. In this work we used thermoluminescence to study S~2/3~Q~B~^β^ and S~2
A thermoluminescence study of the effects of nitrite on photosystem II in spinach thylakoids
β Scribed by Archna Sahay; Anjana Jajoo; Sudhakar Bharti
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 197 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1522-7235
- DOI
- 10.1002/bio.898
No coin nor oath required. For personal study only.
β¦ Synopsis
Abstract
The site of action of nitrite on PS II was investigated by measuring the TL profile of nitriteβtreated spinach thylakoid membranes. Three bands were observed in control, which were identified as the Q band (7Β°C), the B band (24Β°C) and the C band (57Β°C). In the presence of 20 mmol/L nitrite, the intensity of the Q band decreased, the B band upshifted to 46Β°C but the C band disappeared. The suppression of the Q band and the upshift of the B band suggested that nitrite caused inhibition at the water oxidizing complex. The effects of nitrite also remained the same in the presence of chloride. In case of ionβsufficient thylakoid membranes, nitrite decreased the Q band peak intensity and caused an upshift in the B band peak temperature. Nitrite showed similar effects in the presence of DCMU. This suggested that the site of action of nitrite is not at the acceptor side but at the donor side of PS II. The inhibition shown by nitrite has been found to be specific for nitrite anion. No other anions such as formate, fluoride or nitrate, were effective. Copyright Β© 2006 John Wiley & Sons, Ltd.
π SIMILAR VOLUMES
A simple kinetic model was developed for describing nitrite oxidation by autotrophic aerobic nitrifiers in a continuous stirred tank reactor (CSTR), in which mixed (suspended and attached) growth conditions prevail. The CSTR system was operated under conditions of constant nitrite feed concentration
## Abstract A prospective study was made of changes in the electroencephalogram after the use of metrizamide for myelography and posterior fossa cisternography in 61 patients (62 studies). The EEG changed in 21 patients (34%); 3 of these had had previously abnormal records, and the abnormalities we