A thermodynamic framework for the magnesium-dependent folding of RNA
β Scribed by Vinod K. Misra; Ross Shiman; David E. Draper
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2003
- Tongue
- English
- Weight
- 999 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
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We thank Rui Sousa for the gift of the Y639F T7 RNA polymerase plasmid, Kara Juneau and Tom Cech for the DC209 P4-P6 plasmid, Cecilia Cortez and Shirshendu Deb for assistance with nucleoside synthesis, Joe Olvera for technical assistance, and Cheryl Small for assistance with manuscript preparation.
DNA-dependent RNA polymerase has been found to be preferentially released at 43 Β° C from the folded nucleoids of an E. coli dnaA ts mutant when compared with the same nucleoids at 30 Β° C or with nucleoids of a dnaA + strain at either 30 Β° or 43 Β° C. The polypeptides released are identical in molecul