Temperature dependent release of ββ′ subunits of DNA dependent RNA polymerase from the folded chromosome of a dnaA ts mutant of Escherichia coli

✍ Scribed by Eaton, Leslie C. ;Sevall, Jack S. ;Fralick, Joe A.

• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 658 KB
• Volume: 175
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00425527

✦ Synopsis

DNA-dependent RNA polymerase has been found to be preferentially released at 43 ° C from the folded nucleoids of an E. coli dnaA ts mutant when compared with the same nucleoids at 30 ° C or with nucleoids of a dnaA + strain at either 30 ° or 43 ° C. The polypeptides released are identical in molecular weight with those of the fl and/3' constituent polypeptides of the core enzyme of a known E. coli RNA polymerase. In addition, these polypeptides are precipitated by specific anti-RNA polymerase rabbit IgG. The implications of the interactions of RNA polymerase with the dnaA gene product are discussed.