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A Theoretical Experimental Method to Determine the Locus of Desensitization

โœ Scribed by E. Buchman; H. Parnas

Publisher
Springer
Year
1999
Tongue
English
Weight
320 KB
Volume
61
Category
Article
ISSN
1522-9602

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โœฆ Synopsis

Consider a ligand-gated channel with n agonist binding sites which can undergo desensitization. We present a theoretical experimental procedure for pinpointing the principal receptor state from which there is a transition to the desensitized state. The method is based on the observation that the dependence of the slope of the time constant of desensitization vs agonist concentration, at low concentrations, represents the state from which desensitization occurs. In those receptors where desensitization occurs from the open state (or the one immediately preceding it), the method also enables us to determine the number of binding sites.

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