𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A temperature-jump kinetic study of the binding of proflavine to poly I·poly C

✍ Scribed by Charles Steenbergen Jr.; Scott C. Mohr

Publisher
Wiley (John Wiley & Sons)
Year
1973
Tongue
English
Weight
539 KB
Volume
12
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The kinetics of interaction between proflavine and poly I.poly C at 25°C, neutral pH, and moderate ionic strength have been studied by relaxation methods. The qualitative features of this system resemble those previously reported by Crothers and co‐workers for proflavine–DNA and proflavine–poly A·poly U interactions–two relaxations are observed coresponding to a fast bimolecular step followed by a slower isomerization. These results can best be accommodated by a two‐step mechanism leading from the free dye through an “outside‐bound” complex to the intercalated complex. Quantitative comparison of the various rate constants for proflavine binding to different double‐helical polynucleotides shows that the rates are slower for both ribohomopolymer pairs than for DNA. The rates for poly I·poly C are approximately three times faster than these for poly A·poly U.

📜 SIMILAR VOLUMES

Proton transfer involving nucleic acids:
Proton transfer involving nucleic acids: A temperature-jump study of the systems sulphonephthaleins–double stranded poly(A) and sulphonephthaleins–double stranded poly(C)
✍ R. Maggini; F. Secco; M. Venturini; H. Diebler 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 180 KB

The kinetics of proton transfer between poly(A -AH) (partially protonated doublestranded polyadenylic acid) and CPR (chlorophenol red), and between poly(C -H -C) (partially protonated double-stranded polycytidylic acid) and the indicators CPR, BCP (bromocresol purple), and BCG (bromocresol green) ha

Competitive cooperative bindings of a sm
Competitive cooperative bindings of a small ligand to a linear polymer. II. Investigations on the mechanisms of proflavine binding to poly(A) and DNA
✍ M. Dourlent; J. F. Hogrel 📂 Article 📅 1976 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 667 KB

## Abstract Experimental binding isotherms relative to the interactions between proflavine and poly(A) or DNA are analyzed by comparison with theoretical models dealing with competitive cooperative bindings. In the case of poly(A), there are apparently no specific binding sites for the positive co

Kinetics of Demixing and Remixing Transi
Kinetics of Demixing and Remixing Transitions in Aqueous Solutions of Poly(N-isopropylacrylamide): A Temperature-Jump 1H NMR Study
✍ Pavel V. Yushmanov; István Furó; Ilias Iliopoulos 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 141 KB

## Abstract Summary: The time course of the coil‐to‐globule collapse and intermolecular aggregation of poly(__N__‐isopropylacrylamide) in aqueous solution upon exceeding the lower critical solution temperature (LCST) are investigated by temperature‐jump ^1^H NMR spectroscopy. After the temperature

Kinetic study of cooperative binding rea
Kinetic study of cooperative binding reaction of toluidine blue to poly(α,L-glutamic acid) by means of the electric-field pulse method
✍ Hidetoshi Ushio; Tatsuy Yasunaga; Takayuki Sano; Yoshikuni Tsuji 📂 Article 📅 1976 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 665 KB

## Abstract Interaction of toluidine blue with helical and randomly coiled poly(α,L‐glutamic acid) was studied with absorption spectra, titration, and electric‐field pulse measurements. The obtained values of various parameters for the helical form of poly(α,L‐glutamic acid)differed from those for