Kinetic study of cooperative binding reaction of toluidine blue to poly(α,L-glutamic acid) by means of the electric-field pulse method
✍ Scribed by Hidetoshi Ushio; Tatsuy Yasunaga; Takayuki Sano; Yoshikuni Tsuji
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1976
- Tongue
- English
- Weight
- 665 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Interaction of toluidine blue with helical and randomly coiled poly(α,L‐glutamic acid) was studied with absorption spectra, titration, and electric‐field pulse measurements. The obtained values of various parameters for the helical form of poly(α,L‐glutamic acid)differed from those for its coiled form. The difference of parameters in these two forms of poly(α,L‐glutamic acid) was attributed to differences of the binding mechanism in both forms. Furthermore, the binding of toluidine blue to poly(α,L‐glutamic acid) was considered to be due to hydrogen binding in the helical conformation and ionic interaction in the coiled conformation of the polymer.