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A technique for the rapid removal of proteins from cell extracts containing polysaccharides

โœ Scribed by R. G. Beri; J. E. Rollings

Book ID
104648657
Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
256 KB
Volume
9
Category
Article
ISSN
0951-208X

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A rapid and inexpensive assay for dye-binding proteins has been developed. It depends on the separation of free and protein-bound sulfobromophthalein in I-ml columns of Sephadex G-25 due to differential adsorption of the dye to the protein and to the Sephadex. With bovine serum albumin the calibrati