## Abstract Preincubation of sperm in medium for three hours before mixing with ova increased the number of ova fertilized after one hour, thus indicating that sperm maturation occurs in vitro. The presence of cumulus cells increased the proportion of ova fertilized as observed at two and at three
A Technique for Studying Sperm Maturation in vitro
β Scribed by ORGEBIN-CRIST, M. C.; TICHENOR, P. L.
- Book ID
- 109683503
- Publisher
- Nature Publishing Group
- Year
- 1972
- Tongue
- English
- Weight
- 212 KB
- Volume
- 239
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/239227a0
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Garter snake (Thamnophis sp.) embryos of different embryonic ages were explanted from pregnant females and grown in vitro for up to 35 days. The culture medium consisted of Eagle's Minimum Essential Medium with various organic and inorganic supplements and antibiotics. Cultured embryos were injected
## Abstract Canine ovarian oocytes were cultured in a medium consisting of TC medium 199, fetal calf serum and antibiotics. Ninetyβnine percent of the apparently healthy oocytes were in the germinal vesicle (dictyate) stage when recovered from the ovaries; 25% of them reached metaphase I or II by 7