A method is described whereby sedimentation velocity is combined with equilibrium dialysis to determine the net charge (valence) of a protein by using chromate as an indicator ion for assessing the extent of the Donnan redistribution of small ions. The procedure has been used in experiments on bovin
A technique for positioning the ultraviolet light source of the analytical ultracentrifuge
β Scribed by Robert H. Pezzell; Lyndon L. Larcom
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 170 KB
- Volume
- 105
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
A new method is described for accurately positioning the light source of the ultraviolet absorption optical system of the Beckman Model E analytical ultracentrifuge. This method provides very accurate determination of both the height and radial position. A six-channel centerpiece is used to produce a three-line image on the film. Densitometer scans of exposures made at several positions of the source are used to determine the optimum position.
π SIMILAR VOLUMES
Bilayers comprising two matched layers of dielectric thin-( ) film helicoidal bianisotropic mediums TFHBMs with opposite structural handedness can be designed to almost perfectly reflect arbitrarily polarized normally incident plane wa¨es at certain frequencies. TFHBM bilayers can therefore ser¨e as