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A targeted proteomics toolkit for high-throughput absolute quantification of Escherichia coli proteins

✍ Scribed by Batth, Tanveer S. (author);Singh, Pragya (author);Ramakrishnan, Vikram R. (author);Sousa, Mirta M.L. (author);Chan, Leanne Jade G. (author);Tran, Huu M. (author);Luning, Eric G. (author);Pan, Eva H.Y. (author);Vuu, Khanh M. (author);Keasling, Jay D. (author);Adams, Paul D. (author);Petzold, Christopher J. (author)

Book ID
126536421
Publisher
Academic Press Inc.
Year
2014
Tongue
English
Weight
1008 KB
Volume
26
Category
Article
ISSN
1096-7176

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## Abstract Extracellular production of recombinant proteins in __Escherichia coli__ has several advantages over cytoplasmic or periplasmic production. However, nonpathogenic laboratory strains of __E. coli__ generally excrete only trace amounts of proteins into the culture medium under normal grow