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A TaqMan PCR assay using degenerate primers for the quantitative detection of woodchuck hepatitis virus DNA of multiple genotypes

โœ Scribed by Zhuhui Huang; Victor E. Buckwold


Book ID
116735810
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
229 KB
Volume
19
Category
Article
ISSN
0890-8508

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Since the characterization of the genome of the hepatitis E virus (HEV) in 1990, a large genetic diversity has been described. A single real-time reverse transcription (RT)-PCR assay with TaqMan technology has been validated which uses only one set of primers and probe within the ORF2 HEV region (nt