A-T rich sequences in vertebrate DNA

✍ Scribed by John E. Mayfield; Julie F. McKenna

• Book ID: 104708406
• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 565 KB
• Volume: 67
• Category: Article
• ISSN: 0009-5915
• DOI: 10.1007/bf00293173

✦ Synopsis

Partially denatured DNAs from mouse, cow, and chicken were visualized in the electron microscope by the basic protein film technique and the size and distribution of the denatured regions characterized. A-T rich sequences visualized at 15% denaturation average about 1500 bases in length for all three species and are arranged quite non-randomly in the genome. This arrangement is such that 30--50% of the entire genome contains no A-T rich DNA, and another 20% is composed about one-half of A-T rich sequences and one-half of other sequences. Comparison with DNA denaturation profiles indicates that for each organism these sequences are from 25--30% G+C and that there is very little if any DNA more A-T rich than these. Estimates from published studies of fluorescence enhancement of quinacrine bound to A-T rich DNAs suggest that the observed non-random organization of A-T rich sequences is sufficient to account for Q banding of metaphase chromosomes.