A · T → C · G transversions and their prevention by the Escherichia coli mutT and mutHLS pathways
✍ Scribed by Schaaper, Roel M. ;Bond, Barbara I. ;Fowler, Robert G.
- Book ID
- 104688927
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 938 KB
- Volume
- 219
- Category
- Article
- ISSN
- 0026-8925
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📜 SIMILAR VOLUMES
The Escherichia coli mutator gene mutT, which causes A:T----C:G transversion, was cloned in pBR 322. mutT+ plasmids carry a 0.9 kb PvuII DNA fragment derived from the E. coli chromosome. Specific labelling of plasmid-encoded proteins by the maxicell method revealed that mutT codes for a polypeptide
Escherichia coli K12 strain KS40 and plasmid pKY241 were designed for easy screening of supF mutations in plasmid pZ189. KS40 is a nalidixic acid-resistant (gyrA) derivative of MBM7070 (lacZ(am)CA7020). Using in vitro mutagenesis, an amber mutation was introduced into the cloned gyrA structural gene