A systematic approach to the suppression of J cross peaks in 2D exchange and 2D NOE spectroscopy
✍ Scribed by M. Rance; G. Bodenhausen; G. Wagner; K. Wüthrich; R.R. Ernst
- Publisher
- Elsevier Science
- Year
- 1985
- Weight
- 858 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0022-2364
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## Abstract A procedure for the estimation of the mixing time between the last two 90° pulses in the classic three‐pulse sequence NOESY/EXSY is proposed and tested and some considerations for the treatment of the two‐dimensional (2D) ^1^H NMR exchange spectra are given. The rate constants are thus
The splitting pattern of the P-H5" (and P-H5') cross-peak in a 2D 31P-1H correlated NMR spectrum is analysed. Since this cross-peak pattern is strongly dependent on two coupling constants, 'J(PH5") and 3J(H4H5"), it is an efficient probe for the backbone torsion angles fl and y in nucleic acids. A m