A synthetic peptide derived from staphylokinase enhances plasminogen activation by tissue-type plasminogen activator
β Scribed by K. OKADA; S. UESHIMA; H. MATSUNO; N. NAGAI; N. KAWAO; M. TANAKA; O. MATSUO
- Book ID
- 109154717
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 537 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1538-7933
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Molecular assembly of plasminogen and tissue-type plasminogen actwator (t-PA) at the surface of fibrin results in the generation of fibrin-bound plasmin and thereby in the dissolution of a clot. This mechanism is triggered by specific interactions of intra-chain surface lysine residues in fibrin wit
A method is described for determining the activity of recombinant human tissue-type plasminogen activator (rt-PA) by turbidimetry using a microcentrifugal analyzer (MCA). A mixture of thrombin and rt-PA is centrifuged into a mixture of fibrinogen and plasminogen to initiate clot formation and subseq