𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A survey of methylated candidate tumor suppressor genes in nasopharyngeal carcinoma

✍ Scribed by Myriam Loyo; Mariana Brait; Myoung S. Kim; Kimberly L. Ostrow; Chunfa C. Jie; Alice Y. Chuang; Joseph A. Califano; Nanette J. Liégeois; Shahnaz Begum; William H. Westra; Mohammad O. Hoque; Qian Tao; David Sidransky

Publisher
John Wiley and Sons
Year
2011
Tongue
French
Weight
436 KB
Volume
128
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Nasopharyngeal carcinoma (NPC) is a rare malignancy with unique genetic, viral and environmental characteristic that distinguishes it from other head and neck carcinomas. The clinical management of NPC remains challenging largely due to the lack of early detection strategies for this tumor. In our study, we have sought to identify novel genes involved in the pathogenesis of NPC that might provide insight into this tumor's biology and could potentially be used as biomarkers. To identify these genes, we studied the epigenetics of NPC by characterizing a panel of methylation markers. Eighteen genes were evaluated by quantitative methylation‐specific polymerase chain reaction (PCR) in cell lines as well as in tissue samples including 50 NPC tumors and 28 benign nasopharyngeal biopsies. Significance was evaluated using Fisher's exact test and quantitative values were optimized using cut off values derived from receiver–operator characteristic curves. The methylation status of AIM1, APC, CALCA, deleted in colorectal carcinomas (DCC), DLEC, deleted in liver cancer 1 (DLC1), estrogen receptor alpha (ESR), FHIT, KIF1A and PGP9.5 was significantly associated with NPC compared to controls. The sensitivity of the individual genes ranged from 26 to 66% and the specificity was above 92% for all genes except FHIT. The combination of PGP9.5, KIF1A and DLEC had a sensitivity of 84% and a specificity of 92%. Ectopic expression of DCC and DLC1 lead to decrease in colony formation and invasion properties. Our results indicate that methylation of novel biomarkers in NPC could be used to enhance early detection approaches. Additionally, our functional studies reveal previously unknown tumor suppressor roles in NPC.

📜 SIMILAR VOLUMES

Identification of candidate tumor suppre
Identification of candidate tumor suppressor genes inactivated by promoter methylation in melanoma
✍ Vanessa F. Bonazzi; Darryl Irwin; Nicholas K. Hayward 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 400 KB 👁 1 views

## Abstract Tumor suppressor genes (TSGs) are sometimes inactivated by transcriptional silencing through promoter hypermethylation. To identify novel methylated TSGs in melanoma, we carried out global mRNA expression profiling on a panel of 12 melanoma cell lines treated with a combination of 5‐Aza

Functional studies of the chromosome 3p2
Functional studies of the chromosome 3p21.3 candidate tumor suppressor gene BLU/ZMYND10 in nasopharyngeal carcinoma
✍ Wing Lung Yau; Hong Lok Lung; Eugene R. Zabarovsky; Michael I. Lerman; Jonathan 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 French ⚖ 216 KB

## Abstract Chromosome 3p plays an important role in tumorigenesis in many cancers, including nasopharyngeal carcinoma (NPC). We have previously shown chromosome 3p can suppress tumor growth __in vivo__ by using the monochromosome transfer approach, which indicated the chromosome 3p21.3 region was

Identification of Fat4 as a candidate tu
Identification of Fat4 as a candidate tumor suppressor gene in breast cancers
✍ Chao Qi; Yiwei Tony Zhu; Liping Hu; Yi-Jun Zhu 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 French ⚖ 382 KB 👁 3 views

## Abstract __Fat__, a candidate tumor suppressor in __Drosophila__, is a component of Hippo signaling pathway involved in controlling organ size. We found that a ∼3 Mbp deletion in mouse chromosome 3 caused tumorigenesis of a non‐tumorigenic mammary epithelial cell line. The expression of __Fat4__

Spleen tyrosine kinase as a novel candid
Spleen tyrosine kinase as a novel candidate tumor suppressor gene for human oral squamous cell carcinoma
✍ Satoru Ogane; Takeshi Onda; Nobuo Takano; Tetsuo Yajima; Takeshi Uchiyama; Takah 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 French ⚖ 292 KB 👁 1 views

## Abstract We analyzed the mutational and methylation status of the __spleen tyrosine kinase__ (__Syk__) gene and both mRNA and protein levels in primary oral squamous cell carcinoma (OSCC) and OSCC‐derived cell lines and examined the function of the __Syk__ gene in OSCC‐derived cell lines __in vi

A functional investigation of tumor supp
A functional investigation of tumor suppressor gene activities in a nasopharyngeal carcinoma cell line HONE1 using a monochromosome transfer approach
✍ Yue Cheng; Eric J. Stanbridge; Heidi Kong; Ulla Bengtsson; Michael I. Lerman; Ma 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 276 KB 👁 2 views

Monochromosome transfers of selected chromosomes into a nasopharyngeal carcinoma (NPC) cell line were performed to determine if tumor suppressing activity for NPC mapped to chromosomes 9, 11, and 17. Current information from cytogenetic and molecular allelotyping studies indicate that these chromoso