A suggested test for distinguishing between meat extract and yeast extract
β Scribed by Blench, Ronald 0.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1935
- Weight
- 127 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0368-4075
No coin nor oath required. For personal study only.
β¦ Synopsis
Since slight iilteriitions in nppcnriincc nrc not nlwiiys cnsily cliniinntcd, onc find point slioiilil I)c niiitlc. Altc~iitions in i i l ) p e i i ~t ~i ~~ due to ncw nietliotls niny sometimes siniiilittc clinrnctcrist ics which imply stiilencss wlicn referred to the norniiil stiincl:irds for iced fish. Vor csnniplc, conversion of lii~nioglol)in into ~iietliic~iioglol~i~i occurs very sloivly in ice, itncl is, tliercfore, iissociiitctl wit11 :I fairly ntlv:inc*ctl stiigc of tleterioriition. Contiict wit11 c:irbon tliosiclc or with the siilt picked 111) in I)rinr-frcezing :iccrlrriitc~s this rriiction, tlioiigli in both t w c s tlie period of frcslint.ss is incrciisetl. 'I'lic noriiiiil stiiiitliir~l is tliiis no longer nppliciilh. Agiiin, * * rcd iilong tlic
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A simple and reliable method for the efficient inactivation of metabolism and for quantitative metabolite extraction from yeast cells is presented. It is based on the use of a boiling solution made of 75% ethanol (volume/final volume) buffered with 70 mM-Hepes (final concentration), pH 7.5, to guara